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Figure 6 | Journal of Translational Medicine

Figure 6

From: Protein kinase activity is associated with CD63 in melanoma cells

Figure 6

Phosphoamino acid analysis of proteins co-precipitated with the CD63 mAb AHN-16 and the anti-MCSP mAb 9.2.27, and labeled in an in vitro kinase assay. Cells were solubilized, immunoprecipitated, labeled with [γ32P-]ATP, and the resulting phosphoproteins were resolved by SDS-PAGE as in Fig 5. Phosphoproteins were transferred to Immobilon by electroblotting, visualized by autoradiography, excised, and subjected to acid hydrolysis. The resultant phosphoamino acids were resolved by thin layer chromatography and visualized by autoradiography as described in the text. The positions of migration of authentic phosphoserine (P-S), phosphothreonine (P-T), and phosphotyrosine (P-Y) are indicated by dotted lines. A duplicate experiment gave similar results. The lane numbers correspond to the phosphoproteins numbered in figure 5.

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