Figure 2
From: Protein kinase activity is associated with CD63 in melanoma cells
Melanoma cell adhesion to immobilized CD63 mAb was inhibited by Fab fragments. Plates were coated with 0.1 ug/ml of the CD63 mAb AHN-16 as described in Materials and Methods. Cells were preincubated with various concentrations of Fab fragments of AHN-16 (open circles) or of normal mouse IgG (closed circles) for 20 min before adding the cells to the plates for the cell adhesion assays. Cell adhesion assays were performed for 30 min. Results are shown as percent adhesion +/- SEM. A duplicate experiment gave similar results.