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Figure 4 | Journal of Translational Medicine

Figure 4

From: Synergistic inhibition of human melanoma proliferation by combination treatment with B-Raf inhibitor BAY43-9006 and mTOR inhibitor Rapamycin

Figure 4

Phosphorylation of mTOR targets in VMM18 melanoma cells treated with rapamycin, BAY43-9006, or U0126. The phosphorylation of p70S6K and 4EBP1 was assayed by migration in SDS-PAGE and the proteins detected by immunoblotting. Control VMM18 cells grown in PBS without serum were analyzed in lane 1 and VMM18 melanoma cells in lanes 2–6 were grown 24 hours in media that contained 5% serum. Cells not treated with drugs (lanes 1 and 2) were compared to cells pre-treated for one hour with rapamycin (lane 3), BAY43-9006 (lane 4), a combination of rapamycin and BAY43-9006 (lane 5), or U0126 (lane 6). GAPDH was immunoblotted as a loading control (bottom panel).

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Journal of Translational Medicine

ISSN: 1479-5876

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