Figure 3

After stimulation of EC monolayers with 0.1 or 1.0 ng/ mL, IL-1β, cells were incubated with or without blocking TF-antibodies before addition of Factor VIII-deficient plasma. While permeability increased substantially in the absence of TF-blocking antibodies it could be complete abrogated by blocking TF receptors (c) demonstrating the necessity of endothelial TF activity and activation of the extrinsic coagulation cascade to induce permeability. Results were conducted in triplicate.