Transfection of immature DCs with EGFP mRNA. iDCs were transfected by lipofection using DMRIE-C and in vitro-transcribed EGFP mRNA. A) 2.5 μg EGFP mRNA/0.5 × 106 iDCs were transfected and the DCs were kept either as immature cells or matured using a cytokine cocktail. B) 2.5 μg and 5.0 μg EGFP mRNA/0.5 × 106 iDCs, respectively, were transfected and the DCs matured. EGFP expression was analyzed by flow cytometry 48 h after transfection (grey histograms). DCs transfected with H2O only were used as a control (broken line). C) EGFP-expression in transfected DCs was visualized using a fluorescent microscope.