IFN-γ mRNA transcript production by qRT-PCR. The ability of the three CMV peptides; pp65341–349, pp65341–350, and pp65340–355 to reactivate an immune T lymphocyte response after their cognate re-stimulation of self-induced PBMCs (A) or after restimulation with each peptide of 16-mer induced PBMCs (B) was analyzed by qRT-PCR. Levels of IFN-γ mRNA transcript were quantitated after a 3-hour peptide induction. The results were performed in triplicate and reported as the average. Levels of IFN-γ mRNA transcript were normalized by levels of β-actin mRNA transcript and the final values were indicated as the ratio over the negative control.