Table 2 Cytoplasmic markers expressed by primary cultures of skeletal muscle cells grown in vitro for 35 to 40 days1.
Monoclonal antibody | Percentage of positive cells (mean ± SD)2 | Fluorescence pattern |
|---|---|---|
Desmin (skeletal muscle) | 49.6 + 6.4 | Cytoplasmic |
Skeletal myosin type I slow heavy chain (skeletal and cardiac muscle) | 33.4 + 3.9 | Cytoplasmic filaments |
A-sarcomeric actin (skeletal and cardiac muscle) | 28.6 + 6.1 | Cytoplasmic filaments |
Skeletal myosin type II fast heavy chain (skeletal muscle, not cardiac) | 29.4 + 6.2 | Cytoplasmic filaments |
A-sarcomeric actinin (skeletal and cardiac muscle) | 19.0 + 3.7 | Cytoplasmic filaments |
Spectrin (erythrocyte and muscle) | 32.6 + 7.5 | Peripheral rim and membrane staining |
Dystrophin (skeletal, cardiac and smooth muscle) | 38.4 + 9.8 | Peripheral rim and membrane staining |