CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

Table 3 Transwell Experiments

Sorted Immature * or mature CD56+ cells** or K562 were incubated 10:1 ratio in the upper chamber at of Transwell plates while K562 were incubated in the lower chamber. After 2 days, lower K562 were collected labeled overnight with 3H-TDR and analyzed in a β-counter 0.4 μm polycarbonate transwell membrane were removed and the upper compartment cells were harvested and evaluated for viability by trypan blue exclusion assay.

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