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Table 3 Transwell Experiments

From: CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines

  3H-TDR (CPM)
  *Immature CD56 **Mature CD56 ***K562
Exp 1 6781 ± 51 5941 ± 560 4429 ± 197
Exp 2 3116 ± 65 2534 ± 196 4030 ± 239
Exp 3 6187 ± 512 2932 ± 181 6873 ± 34
  1. Sorted Immature * or mature CD56+ cells** or K562 were incubated 10:1 ratio in the upper chamber at of Transwell plates while K562 were incubated in the lower chamber. After 2 days, lower K562 were collected labeled overnight with 3H-TDR and analyzed in a β-counter 0.4 μm polycarbonate transwell membrane were removed and the upper compartment cells were harvested and evaluated for viability by trypan blue exclusion assay.