|
3H-TdR (CPM)
| |
---|
|
ImmatureCD56+ cells + K562
|
K562 only
|
% proliferation inhibition
|
---|
1
|
214
|
6183.5
|
96.5
|
2
|
267
|
6183.5
|
95.7
|
3
|
8183
|
11172
|
27
|
4
|
1805
|
5283
|
66
|
5
|
1944.5
|
4720
|
59
|
6
|
743
|
11232
|
93.4
|
7
|
2708
|
10323
|
74
|
8
|
355
|
2524
|
86
|
9
|
138
|
2524
|
94.6
|
10
|
829
|
7336
|
89
|
Mean ± SD
|
1718.7 ± 2433.5
|
6748.1 ± 3255.5
|
78 ± 22.2
|
- Electronically or magnetically sorted CD34-derived CD56+ cells and incubated 10:1 ratio with K562 and cell proliferation was evaluated after 2-day culture. The difference in the mean CPM incorporation between immature CD56+cells+K562 and K562 alone was highly significant with a P value of 0.0011. The range of CPM incorporation in the presence of immature CD56+ cells and K562 was 138–8103 while in the presence of K562 alone was 2524–11232.