Is Melanoma a stem cell tumor? Identification of neurogenic proteins in trans-differentiated cells

Table 2 Higher Detection Frequency in Neuronal Cells

Protein Name	Abbreviation	Accession #	Functional Category
Alkaline Phosphatase	PPBT	Q29486	Membrane Phosphatase
Aminopeptidase N	AMPN	P79171	Membrane Enzyme
Arylsulfatase B precursor	ARSB	P33727	Lysosomal Enzyme
Brain-derived neurotrophic factor precursor	BDNF*	Q9TST3	Neurotrophic Factor
Cellular tumor suppressor p53	P53*	P41685	Nuclear
Dipeptidyl peptidase IV	DPP4	Q9N2I7	Membrane Enzyme
Glutamate decarboxylase, 67 kDa isoform	DCEI	P14748	Cytoplasmic Enzyme
Integrin beta-1 precursor	ITB1	P53713	Membrane Receptor
Interleukin-1 beta convertase precursor	ICE1	Q9MZV6	Cytoplasmic Enzyme
Lysosomal alpha-mannosidase precursor	M2B1	O46432	Lysosomal Enzyme
Major prion protein precursor	PRIO	O18754	Membrane Prion Protein
Mast/stem cell growth factor receptor precursor	KIT	Q28889	Membrane Tyrosine Kinase Receptor
NADH-Ubiquinone oxidoreductase chain 5	NU5M	P48921	Mitochondrial Enzyme
Polycomb complex protein BMI-1	BMI1	Q9TST0	Nuclear Protein
Pyruvate kinase, M1 isozyme	KPYM	P11979	Pyruvate Kinase
Serum albumin precursor***	ALBU*	P49064	Cytoplasmic Albumin Protein
Sodium/calcium exchanger 1 precursor	NAC1	P48767	Membrane Calcium Ion Channel (Plasma Membrane)
T-cell surface glycoprotein CD8 beta chain precursor	CD8B	P79336	Membrane Receptor
Toll-like receptor 4 precursor	TLR4	P58727	Membrane Receptor
Transferrin receptor protein 1	TFR1	Q9MYZ3	Membrane Receptor
Zona pellucida sperm-binding protein 2 precursor	ZP2*	P47984	Membrane Receptor
Zona pellucida sperm-binding protein B precursor	ZP4	P48834	Membrane Receptor

Proteins with higher frequency of detection in complex forms in neuronal cells compared to melanoma. Ten proteins were detected in single as well as in complex forms in both melanoma and neuronal cells. These proteins displayed a higher frequency of detection in neuronal cells than in melanoma. Four proteins Fel d 2 /ALBU*, BDNF*, tumor suppressor protein (P53*) and zona pellucida-2 (ZP2*) were detected both in single (*) and complex forms only in neuronal cells. These proteins were present only in complexes in melanoma cells. The ALBU and P53* proteins also showed increased frequency of detection in the neuronal cells than in melanoma (also see Fig 2C*) and BDNF and ZP2 showed similar detection frequencies in the two cell types.

ISSN: 1479-5876