Lymphocyte recruitment and activation in patient#12's melanoma. T cells that were double-stained by anti-CD3 and anti- CD57 mAb were counted in 2 sections of 6 lymph nodes available from the lymph node dissection specimen obtained before (A. April 2001) and after (B. July 2002) treatment (cf Fig. 3). Counts were performed in 12 sections in total as follows: cells that were double-stained by CD3 and CD57 were counted on the whole section including B cell areas, and the total count was reported to 1 mm2. Results showed an increase of the CD3+CD57+ cells after treatement (mean = 122/mm2) as compared to the count before treatment (mean: 58/mm2).