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Figure 5 | Journal of Translational Medicine

Figure 5

From: Human primary osteoclasts: in vitro generation and applications as pharmacological and clinical assay

Figure 5

Dose response curves for zoledronic acid, raloxifene, E-64 and AAR494 in the human osteoclast assay: measurements of osteoclast formation, pit area and collagen fragment release. Human osteoclasts were generated as described under Materials and Methods in the presence of increasing concentrations of compounds, as indicated. At the end of the 17 day-culture three quantitative read-outs were measured: the number of TRAP-positive multinucleated cells on the plastic, the resorbed area on bone slices, and the concentration of collagen fragments in the medium. The graphs were generated with Sigma Plot, and concentrations inducing 50% inhibition (IC50 values) were calculated after curve fitting by the software Origin 7. The following IC50 values are means ± SEM from 3 independent dose response curves, in which each measurement was done in quadruplicates. The IC50 values are shown in the order: cell number, resorbed area, and collagen fragments: zoledronic acid (102 ± 4; 173 ± 127, 182 ± 150 nM), raloxifene (17 ± 3; 29 ± 13; 46 ± 13 nM), E-64 (>1000; 17 ± 2; 4 ± 3 nM), and AAR494 (>1000; 127 ± 105; 56 ± 27 nM).

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