Establishment of a collagen fragment release read-out with dentin and bone slices. Collagen fragments released from the pits on dentin or bone slices were measured in the cell culture medium with CrossLaps ELISA kit. The medium was collected after 3 days of cell culture at the indicated times (for example, day 10 contains medium from days 7–10). The cultures were treated from the start with the vehicle PBS or with 50 nM zoledronic acid. The numbers over the bars for treated samples are % inhibition relative to the corresponding control.