Figure 3From: Epitope definition by proteomic similarity analysis: identification of the linear determinant of the anti-Dsg3 MAb 5H10Identification of the epitopic sequence recognized by mouse anti-Dsg3 1–212 MAb 5H10. Dot-blot immunoassay was performed on nitrocellulose membrane spotted with human Dsg3 (10μg), or Dsg3 peptide (2.5μg) corresponding to the sequence: 1) Dsg336–45EEMTMQQAKR; 2) Dsg349–60REWVKFAKPCRE; 3) Dsg3173–187NSLVMILNATDADEP.Back to article page