Figure 3

CD8⁺ effector T cells can be hyperexpanded through repetitive anti-CD3 stimulation. (A) CD62L^low TDLN cells were restimulated with anti-CD3 mAb for 14 hrs every 7 days starting on day 23 of culture and overall proliferation was measured for three independent experiments. (B) T cells were harvested on day 50 of culture and adoptively transferred to hosts bearing either MCA 205 or MCA 207 3-day pulmonary metastases (P < 0.01 for MCA 205 tumors treated with either 6 × 10⁶ or 2 × 10⁷ compared with all other groups). (C) Mice bearing 3-day s.c tumors were treated with 5 Gy TBI then received adoptive transfer of the indicated number of T cells hyperexpanded for 50 days (P = 0.06 for 4 × 10⁷ cell dose) (D) Mice bearing 3-day i.c. tumors were treated with 5Gy TBI then received adoptive transfer of the indicated number of T cells hyperexpanded for 50 days. Mice were sacrificed when they developed neurologic symptoms indicating progressive tumor (P = 0.9 for treatment groups versus control).