Expression of EphA2 in murine tumor cell lines and tumor/normal tissues. (A) Aliquots of protein lysates (10 μg/lane) were analyzed by Western Blotting for expression of mEphA2 protein using a specific monoclonal antibody (C-20 Ab; Santa Cruz Biotechnology, Inc., Santa Cruz, CA). Lysate samples were obtained from mouse tumor lines including the B16, B16V1 and B16BL6 melanomas and the KR129, KR130, KR233 and KR158D glioma cell lines derived from spontaneously developed glioma tissues in NPcis mice. Control β-actin labeling demonstrated equal amount of protein loading in each lane. (B) Protein lysates isolated from normal mouse brain, spleen, liver, lung, heart, skeletal muscle, and the G261 glioma and MCA 205 sarcoma cell lines were separated by SDS-PAGE, blotted onto nitrocellulose and analyzed for expression of mEphA2 using C-20 anti-EphA2 monoclonal antibody.