Figure 5From: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assayLES/LM609 pathway. (A) Alpha v beta 3 staining was most intense in stimulated CAM disks. The difference in staining was found to be statistically significant between stimulated CAM disks and disks treated with fumagillin (P = 0.01, Student's t test). (B) Cleaved caspase-3 staining was found to be greatest in stimulated disks treated with carrier solution and least in CAM disks treated with fumagillin (P = 0.007, Student's t test) and LM609 (P = 0.014, Student's t test).Back to article page