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Figure 2 | Journal of Translational Medicine

Figure 2

From: A novel technique for quantifying changes in vascular density, endothelial cell proliferation and protein expression in response to modulators of angiogenesis using the chick chorioallantoic membrane (CAM) assay

Figure 2

Evaluation of CAM photomicrographs by blinded grader evaluation. (A) Results of blinded grading evaluation showed no difference between the three groups tested. (B) Confocal fluorescent microscopy evaluation of CAM disks. CAM disks were scanned under the Zeiss LSM 5 Pascal confocal fluorescent microscope using the FITC filter at 488 nm. A significant difference was measured by mean fluorescent vascular density (+/- SEM) between unstimulated CAM disks and stimulated CAM disks after systemic injection of a carrier solution (P = 0.004, Student's t test) and between stimulated CAM disks which received systemic injection of fumagillin and carrier solution P = 0.015, Student's t test).

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