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Table 1 Comparison between different methods for gene silencing.

From: RNA interference: learning gene knock-down from cell physiology

Method Advantages Drawbacks
RNA interference Specific
Relatively easy
Knock-down (not knock-out)
Needs transfection
Anti-sense DNA Easy
Inexpensive
Variable efficiency
Variable specificity
Needs transfection
Dominant negative mutants Stable suppression
Specific protein domains can be targeted
Needs transfection
Variable/unexpected effect
Knock-out animal Complete gene silencing Labor intensive, expensive
Lethal mutants may prevent embryonic development
Small molecule inhibitors Easy delivery Variable specificity
Labor intensive development