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Table 2 Comparison of the GrB ELISPOT, IFN-γ ELISPOT and 51Cr-release assays for measuring Lymphokine Activated Killer (LAK) cell activity.

From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay

Effector:Target Ratio

51Cr-Release Assaya (% Cytotoxicity ± SE)

IFN-γ ELISPOT Assayb (Spots/well ± SE)

GrB ELISPOT Assayc (Spots/well ± SE)

20:1

42 ± 10

TNTCd

TNTC

10:1

30 ± 10

TNTC

TNTC

5:1

22 ± 6

TNTC

TNTC

2.5:1

10 ± 3

TNTC

TNTC

1:1

5 ± 1

TNTC

TNTC

0.4:1

4 ± 1

227 ± 16

282 ± 18

0.2:1

2 ± 1

182 ± 8

193 ± 12

0.1:1

2 ± 2

121 ± 14

117 ± 12

0.04:1

1 ± 0

61 ± 13

54 ± 11

0.02:1

1 ± 0

29 ± 8

37± 7

  1. a The 51Cr-release assay was performed with the effector:target cell ratio as specified for 4 h at 37°C. Data presented as average cytotoxicity ± SE (n = 3).
  2. b The IFN-γ ELISPOT assay was performed with K562 cells at a constant number of 5 × 104 cells per well and effector:target cell ratio as specified for 24 h at 37°C. Data presented as average spots/well ± SE (n = 3).
  3. cThe granzyme B ELISPOT assay was performed with K562 cells at a constant number of 5 × 104 cells per well and effector:target cell ratio as specified for 4 h at 37°C. Data presented as average spots/well ± SE (n = 3).
  4. dTNTC= Too numerous to count accurately.