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Table 1 Comparison of the GrB ELISPOT and 51Cr-release assays for measuring TALL-104 cell activity.

From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay

Effector:Target Ratio

51Cr-Release Assaya (% Cytotoxicity ± SE)

GrB ELISPOT Assayb (Spots/well ± SE)

10:1

65 ± 4

TNTCc

5:1

57 ± 10

TNTC

2.5:1

47 ± 11

TNTC

1:1

20 ± 5

TNTC

0.5:1

9 ± 2

TNTC

0.2:1

2 ± 1

316 ± 50

0.1:1

2 ± 1

144 ± 13

0.05:1

1 ± 1

80 ± 6

0.02:1

0 ± 1

33 ± 4

  1. a The 51Cr-release assay was performed with the effector:target cell ratio as specified for 4 h at 37°C. Data presented as average cytotoxicity ± SE (n = 3).
  2. bThe granzyme B ELISPOT assay was performed with K562 cells at a constant number of 5 × 104 cells per well and effector:target cell ratio as specified for 4 h at 37°C. Data presented as average spots/well ± SE (n = 3).
  3. cTNTC= Too numerous to count accurately.