Figure 8
From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay
Correlation between CD107a expression measured in the flow cytometric assay and Granzyme B release measured in the ELISPOT assay. Human peripheral blood mononuclear cells (PBMC) were cultured with 100 U/ml of IL-2 for 5–6 days to induce LAK cells. LAK cells (5 × 103 cells per well) were incubated with K562 cells (5 × 104 cells per well) in the Granzyme B ELISPOT and 2 × 105 LAK cells were incubated with 1 × 105 K562 in the CD107a mobilization assay for 0.5, 1 and 3 h. The ELISPOT data are presented as spots per 1.0 × 105 effectors ± SE (n = 9) and the CD107a data as the number of positive cells per 1.0 × 105 effectors ± SE (n = 9). All results are corrected for background. A significant correlation between the number of GrB spots and CD107a positive effector cells was observed (R2 = 0.89).