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Figure 7 | Journal of Translational Medicine

Figure 7

From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay

Figure 7

Kinetics of Granzyme B and IFN-γ secretion by LAK cells in the ELISPOT assays. Human peripheral blood mononuclear cells (PBMC) were cultured with 100 U/ml of IL-2 for 5–6 days to induce LAK cells. LAK cells (5 × 103 cells per well) were incubated with K562 cells (5 × 104 cells per well) in the Granzyme B and IFN-γ ELISPOT assays for 0.2, 0.5, 1, 4 or 24 h at 37°C. Data are presented as average spots per well ± SE (n = 6). Results are corrected for background.

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