Figure 4
From: Evaluating the cytotoxicity of innate immune effector cells using the GrB ELISPOT assay
Effect of Brefeldin A on Granzyme B and IFN-γ secretion in the ELISPOT assays. TALL-104 cells were preincubated with brefeldin A (5 μg/ml; 1 h at 37°C) prior to use in the ELISPOT assays. TALL-104 cells (2.5 × 103 cells per well) were incubated with K562 (5 × 104 cells per well) for 4 h in the Granzyme B and 24 h in the IFN-γ ELISPOT assays at 37°C. Data are presented as percent inhibition ± SE (n = 3). Results are corrected for background.