Figure 5
Cytotoxic T Lymphocyte (CTL) Assay. Splenocytes from C57BL/6 mice were re-stimulated for 6 days with mitomycin C-treated B16-PSMA cells. Cytotoxicity was measured by LDH release assay. The experiment was carried out in triplicate. Shown is the average CTL (n = 6) with standard errors. When B16-PSMA cells were used as target cells, specific lysis was found in DNA vaccination group but not in control group. The CTL reaction was enhanced by CpG oligodeoxynucleotides(Fig. 5A). However, when the mock-transfected B16-pCDNA cells were used as target cells, specific lysis was not observed in all groups (Fig. 5B). * indicated the difference between control group with other two groups was statistically significant (P < 0.05); â–³ indicated the difference between DNA vaccination group and CpG + DNA vaccination group was statistically significant (P < 0.05).