Figure 1From: Co-administration of a DNA vaccine encoding the prostate specific membrane antigen and CpG oligodeoxynucleotides suppresses tumor growthImmunofluorescence staining of COS7 cells. The COS7 cells were transfected with either pCDNA3.1-PSMA or empty pCDNA3.1 and fixed with cold aceton. Fixed cells were incubated with a anti-PSMA monoclonal antibody 4A12, stained with a goat anti-mouse immunoglobulin-FITC conjugate, PSMA immunoreactive cells were visualized with a fluorescent microscope (×40). Cytoplasmatic reactivity was found in COS7 cells transfected with pCDNA3.1-PSMA (A) but not in COS7 cells transfected with pCDNA3.1 (B).Back to article page