Genetic polymorphisms of CYP2A13 and its relationship to nasopharyngeal carcinoma in the Cantonese population

Table 1 Details of the primers used in this study

Primers	primer sequence(5'-3'orientation)	sequence region	Annealing temperature
8KF	tgtcctaagctgtgtgggattcagggttgg	exon 1	62°C
8KR	aaatgggcatgaacgcttttccccttcgt		62°C
Exon 1F	cctgactgtgatggtcttgatgtcagtctgcc		66°C
Exon 1R	agggagttgtacatctgctctgtgttcagctg		66°C
Exon 2F	ctccctcatgaaggtgtccta	intron1 exnon2
Intron 2R	agagggaagcagagagaggag	partial of intron2
Exon 3F	cccctgacctctctccac	Exon3
Exon 4R	actgattttgaggggacactg	Exon4
Exon 5F	cctggacagatgcctttaactccg	Exon5	62°C
Exon 5R	tggctttgcacctgcctgcact		62°C
Exon 6F	cctctttccacctttggtct	Exon 6
Exon 7R	gcaggaagctcatggtgtagt	Exon 7
Exon 8F	catgtctcccaaacttcctgt	Exon 8
Exon 9R	gcaggaagctcatggtgtagt	Exon 9
2A13-RNAF	gggccaagatgccctacatg		68°C
2A13-RNAR	cgtcaatgtccttaggtgactgg		68°C
GAPDH-F	aatcccatcaccatcttcca		62°C
GAPDH-R	cctgcttcaccaccttcttg		62°C

ISSN: 1479-5876