Figure 2From: Genetic polymorphisms of CYP2A13 and its relationship to nasopharyngeal carcinoma in the Cantonese populationMismatch PCR-RFLP and PCR-RFLP assay for analyzing the 74 G>A and 3375 C>T polymorphisms. A: mismatch PCR generats a 132 bp amplicon which was digested by Nae I to fragments of 101 bp and 31 bp for the wild-type amplicon, but the 74 G>A mutatnt allele is uncut. Lane 4: homozygote (74 AA); Lanes 15,16: heterozygote (74 GA). Other lanes are digestion products of wild-type amplicons. B: PCR products of the wild-type 332 bp amplicon containing the 3375 C is digested by Hha I to fragments of 233 bp and 99 bp (lanes 1–6, 8–10). Lane 11: digestion of the homozygous 3375 TT mutant allele. Lane 7: digestion of heterozygote (3375 CT).Back to article page