Figure 5
Tetramer analysis of PBMCs from case 17. Lymphocytes were collected from peripheral blood of the patient reported as case 17 before the 1st vaccination (A) and after the 4th vaccination (B), stained with FITC-labeled antiCD8 mAb and PE-labeled HLA-A24/survivin 2B peptide tetramer, and analyzed by flow cytometry. The frequency of tetramer-positive CTL was increased from 0.09% to 0.35% of CD8+ T cells after the 4th vaccination.