Figure 2From: CD8+ T lymphocyte responses target functionally important regions of Protease and Integrase in HIV-1 infected subjectsCD8 T cell responses to HIV-1 Integrase. PBMC were stimulated with the indicated peptide in an overnight IFN-γ ELISPOT assay. Each row represents an individual peptide. (A) Amino acid sequence, using standard single-letter amino acid abbreviations. Numbers above peptide sequence indicate amino acid position within Integrase. The conserved HHCC zinc finger-like domain and DDE element are indicated in bold. (B) Bars represent the percentage of 56 study subjects who responded to the peptide in an ELISPOT assay. Peptides with the highest number of responses are shaded gray. (C) The magnitude of every CTL response against Integrase peptides detected in the study cohort. Each symbol represents a single CTL response against that peptide by one individual. Magnitude of responses are after subtraction of background, which in all cases was <30 SFC/million PBMC. Symbols are the same as in Figure 1: Closed circle (●): acute cohort. Open circle (○): chronic cohort. Shaded triangle (): controllers.Back to article page