Figure 1

CD8 T cell responses to HIV-1 Protease. PBMC were stimulated with the indicated peptide in an overnight IFN-γ ELISPOT assay. Each row represents an individual peptide. (A) Amino acid sequence, using standard single-letter amino acid abbreviations. Numbers above peptide sequences refer to the amino acid position within Protease, with key Protease inhibitor mutation residues indicated in bold. (B) Bars represent the percentage of 56 study subjects who responded to the peptide in an ELISPOT assay. Peptides with the highest number of responses are shaded gray. (C) The magnitude of every CTL response detected in the study cohort. Each symbol represents a single CTL response against that peptide by one individual. Magnitudes of responses are shown after subtraction of background, which in all cases was <30 SFC/million PBMC. Closed circle (●): acute cohort. Open circle (○): chronic cohort. Shaded triangle (): controllers.