Skip to main content
Figure 3 | Journal of Translational Medicine

Figure 3

From: Identification and characterization of a novel nasopharyngeal carcinoma-associated peptide: NAP-1

Figure 3

NAP-1 protein is a secreted protein secreted by FDC. A: NAP-1 gene is expressed in S100+ CD35+ FDC cell lines 1. In situ hybridization by Dig labelled NAP-1 cDNA probe in FDC lines (×300); 2. Immunohistochemistry by Mouse Anti-S100 Monoclonal antibody in FDC lines (×300); 3. Immunohistochemistry by Mouse Anti-CD35 Monoclonal antibody in FDC lines (×300); 4. Immunohistochemistry by Rabbit Anti-NAP-1 polyclonal antibody in FDC lines (×300) B: Western blotting analysis of FDC culture supernatants using rabbit anti-NAP-1 polyclonal antibody 1. The negative antibody control: the primary antibody replaced with block solution; 2. The negative supernatants control: the FDC lines culture supernatants replaced with fresh media; 3. Western blotting analysis of FDC supernatants cultured for 1 day; 4. Western blotting analysis of FDC supernatants cultured for 2 days

Back to article page