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Figure 1 | Journal of Translational Medicine

Figure 1

From: In vitro biosafety profile evaluation of multipotent mesenchymal stem cells derived from the bone marrow of sarcoma patients

Figure 1

Morphological analysis and in vitro differentiation of mesenchymal stem cells from control (MSC-CTRL) and sarcoma (MSC-SAR) patients. (A) The morphology of MSC-CTRL and MSC-SAR samples with culture passage. Representative bright-field images of MSC-CTRL and MSC-SAR at early (P3) and late (P10) passages. White boxes indicate areas of magnification shown in the lateral panels. Scale bar = 100 μm. (B) Osteogenic differentiation assay of MSC-CTRL and MSC-SAR samples. Representative bright-field images of Alizarin Red staining of non-induced MSC-CTRL and MSC-SAR, and after 14 days in osteogenic medium. Red staining highlights deposition of mineralized extracellular matrix by differentiated MSCs. Scale bar = 100 μm. (C) Adipogenic differentiation assay of MSC-CTRL and MSC-SAR samples. Representative bright-field images of Oil Red O staining of non-induced MSC-CTRL and MSC-SAR and after 21-day culture in adipogenic medium. Red staining indicates fat deposits, a hallmark of fully differentiated adipocytes. Scale bar = 50 μm.

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