Figure 3

The inhibitor effect of MIF on L-type voltage-dependent Ca2+ channels in mouse pancreatic β cells (HIT-T15 cell line). (A) Representative recordings of whole-cell ICa,L are shown for the control cells, and cells treated with 20 nM, 40 nM, or 60 nM rMIF for 24 h. (B) Representative ICa,L density– voltage relationships in control cells (n = 6) and cells treated with 20 nM (n = 6), 40 nM, or 60 nM rMIF (n = 6). (C) A double-pulse protocol is shown with the testing potentials stepped from - 70 to 0 mV at an HP of - 40 mV. The interval between two pulses was 10 ms. An inhibitory effect of MIF on the recovery kinetics from L-type voltage-dependent Ca2+ channels inactivation was observed.