Table 2 Summary of blood sample type differences across 12 multiplexes for 24 subjects
Multiplex designation (Myriad-RBM designation) | Dilution factor | Number of analytes | Comments (Multiplex-specific characteristics) |
|---|---|---|---|
Microalbumin | 1:1E6 | 1 | Simplex, one-analyte only; requires substantial dilution; low reliability across duplicates in all sample types. |
HCVD4 | 1:5 | 5 | 2/5 analytes not detected; serum has better performance for 1/5 analytes. |
HMP8 | 1:200 | 10 | All analytes detected; plasma has better performance for 3/10 analytes; mean levels vary between serum and plasma for 4/10 analytes. |
HMPC19 | 1:5 | 6 | All analytes detected; plasma has better performance for 1/6 analytes; serum has better performance for 2/6 analytes. |
HMPC35 | 1:5 | 7 | 2/7 analytes not detected; plasma has better performance for 1/7 analytes; serum has better performance in 1/7 analytes. |
HMPC42 | 1:5 | 8 | All analytes detected; serum has better performance in 1/8 analytes. |
HMPC49 | 1:5 | 5 | 3/5 analytes not detected consistently; serum has better performance in 1/5 analytes. |
HMPC62 | 1:5 | 6 | 1/6 analytes not reliably detected; serum has better performance for 1/6 analytes; mean levels higher in serum for 3/6 analytes. |
HMPC83 | 1:5 | 9 | All analytes detected in at least one sample type; serum has better performance in 2/9 analytes; mean level higher in serum for 3/9 and higher in plasma for 1/9 analytes. |
HMPC84 | 1:100 | 7 | All analytes detected; sample types behave similarly; mean level higher in serum for 1/7 analytes. |
HMPCORE1 | 1:5 | 17 | 12/17 analytes not detected or not consistently detected; plasma shows better performance for 1/17 analytes (only detected in plasma); serum has better performance for 2/17 analytes; mean level higher in serum for 1/17 analytes. |
HMPCORE2 | 1:5 | 16 | 5/16 analytes not consistently detected in any sample type; 2/16 analytes were only consistently detected in serum; 2/16 analytes performed better in plasma. Mean level higher in serum for 3/16 analytes. |
HMPCORE4 | 1:200,000 | 8 | All analytes detected; plasma has better performance for 4/8 analytes; CV > 10% for a majority of analytes and sample types; higher overall CVs compared to other multi-plexes. Highest dilution of all multiplexes |