The role of quantitative mass spectrometry in the discovery of pancreatic cancer biomarkers for translational science

Table 1 Protein deep mining studies in pancreatic cancer using mass spectrometry

Technique	Sample type	Groups	Discovery phase	Biomarker candidates	References
Label-free	Tissue	PC, N	1,009 proteins in total; 422 upregulated proteins in PC	ASPN, LTBP2, TGFBI	Turtoi et al. [49]
	Tissue	PC, N	1,229 proteins in total; 499 upregulated in PC	ECH1, GLUT1 (GTR1), OLFM4, STML2	Takadate et al. [30]
	Tissue	PC, CP, N	525 proteins in total; 21 upregulated proteins in PC	ANXA4, FN1	Paulo et al. [65]
	Plasma	PC, N	53,009 MS peaks	CXCL7	Matsubara et al. [69]
SILAC	Cell lines	PC, N	195 proteins in total; 68 upregulated proteins in PC; 5 biomarker candidates validated in pancreatic cancer tissue	CD9, HSPG2, APOE, SDF4, ITGB1	Grønborg et al. [81]
	Serum	PC, N	1,065 proteins in total; 121 upregulated proteins in PC	BCAM, ICAM1	Yu et al. [ 94]
ICAT	Tissue	PC, N	656 proteins in total; 151 upregulated proteins in PC	ANXA2, ITGB1	Chen et al. [100]
	Pancreatic juice	PC, N	105 proteins in total; 30 upregulated proteins in PC	IGFBP2	Chen et al. [103]
Acrylamide- labeling	Plasma	PC, CP, N	1,340 proteins in total; 95 and 87 proteins with ≥1.5 fold difference in PC compared to N and CP, respectively	ICAM1, TIMP1	Pan et al. [106]
TMT	Serum	PC, N	752 proteins in total	APOA4, F12, GSN, LTF	Sinclair et al. [110]
ICAT, iTRAQ	Tissue	PanIN-3, PC, CP, N	770 proteins in total; 70 proteins upregulated and 133 downregulated in PanIN-3	ACTN4, LAMB1, LGALS1	Pan et al. [119]

ACTN4, actinin alpha-4; ANXA2, annexin A2; ANXA4, annexin A4; APOE, apolipoprotein E; APOA4, apolipoprotein A-IV; ASPN, asporin; BCAM, basal cell adhesion molecule; CP, chronic pancreatitis; CXCL7, CXC chemokine ligand 7; ECH1, enoyl CoA hydratase 1; F12, coagulation factor XII; FN1, fibronectin 1; GLUT1 (GTR1), glucose transporter member 1; GSN, gelsolin; HSPG2, heparan sulfate proteoglycan 2 (perlecan); ICAM1, intercellular adhesion molecule 1; IGFB2, insulin-like growth factor-binding protein 2; ITGB1, integrin beta-1; LAMB1, laminin beta-1; LGALS1, galectin-1; LTBP2, latent transforming growth factor beta binding 2; LTF, lactotransferrin; N, normal; OLFM4, olfactomedin-4; PanIN-3, pancreatic intraepithelial neoplasia; PC, pancreatic cancer; SDF4, stromal cell derived factor 4; STML2, stomatin-like protein 2; TGFBI, transforming growth factor beta-induced; TIMP1, metalloproteinase inhibitor 1.

ISSN: 1479-5876