Figure 2

Representative immunological monitoring assays detecting peptide-specific CTL response. PBLs obtained from CR patient (case 6) after the 8^th vaccination were cultured with URLC10-peptide stimulation and subjected to the ELISPOT assay (a, b). The cultured lymphocytes were analyzed by flow cytometry, and proportion of URLC10 dextramer positive cells in CD8⁺CD3⁺CD4^- cells were calculated (c).