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Figure 4 | Journal of Translational Medicine

Figure 4

From: Yin Yang 1 contributes to gastric carcinogenesis and its nuclear expression correlates with shorter survival in patients with early stage gastric adenocarcinoma

Figure 4

YY1 ectopic expression enhanced tumor growth both in vitro and in vivo . (A) Western blot of YY1, active-β-catenin and total β-catenin after YY1 exogenous overexpression in AGS, MKN28 and NCI-N87 cell lines. (B) 5-day MTT assays revealed YY1 enhanced gastric cancer cell proliferation (*, p < 0.05). (C) Upper: Western blot of active-β-catenin and total β-catenin under siCTNNB1 treatment. Lower: siCTNNB1 inhibits GAC cell proliferation (**, p < 0.001). (D) YY1 enhanced monolayer colony formation to 2.4, 2.3 and 1.9 folds in AGS, MKN28 and NCI-N87 cells, respectively, compared with vector controls (**, p < 0.001). The experiments were done in triplicates. (E1) YY1 ectopic expression activated Wnt/β-catenin signaling pathway in MKN45 cells by consecutively activating active-β-catenin, CCND1 and c-Myc. (E2) YY1-MKN45 formed bigger xenograft tumors than pcDNA3.1-MKN45 20 days after injection (**, p < 0.001).

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