Figure 3
YY1 knockdown suppressed Wnt/ β -catenin signaling pathway and inhibits tumor growth in vivo. (A) Relative luciferase activity of Wnt/β-catenin signaling pathway in 4 gastric cancer cell lines after siYY1 treatment. (B) Relative luciferase activity of TOPflash in AGS, MKN28, NCI-N87 and MGC-803 cells with siYY1 transfection (*, p < 0.05; **, p < 0.001). (C) Western blot of active-β-catenin, total β-catenin, CCND1 and c-Myc after siYY1 transfection in AGS, MKN28 and NCI-N87 cells. (D1) Western blot of YY1 expression in stable clones of shYY1-MKN45 and the vector control. (D2) shYY1-MKN45 formed smaller xenograft tumors than pBABE-MKN45 30 days after subcutaneous injection (**, p < 0.001).