The neuron-cell-like morphology of AT-MSCs isolated from young, adult and aged donors. (A-C) Neuron-like-morphology was observed in MSCs isolated from young, adult and aged individuals, respectively. (D) Percentage of AT-MSCs showing neuron-like morphology. Representative slide showing nestin expression as determined by immunofluorescence staining (E) with similar expression in all groups (F). Real time RT-PCR analysis showed equivalent up-regulation of neurogenic specific genes (NFM and NSE) in AT-MSCs isolated from donors of different age (G). NSE: Neuron-Specific-Enolase, NFM: Neurofilament.