Figure 4
Effect of donor age on osteogenic potential of AT-MSCs. Osteogenic induction was assessed by von Kossa staining. (A-C) Representative figures showing matrix mineralization in induced cultures of young, adult and aged, respectively. (D-F) Control AT-MSCs did not stain positive with von Kossa staining. Possible age related differences in osteogenic potential were measured using ImageJ software. AT-MSCs isolated from young individuals revealed more matrix mineralization than adult and aged groups (G). Similar, results were obtained when gene expression of OST and ALP was analyzed through quantitative RT-PCR (H). Results are expressed as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001 for young AT-MSCs versus aged AT-MSCs, #P for adult versus aged AT-MSCs. OST: osteocalcin, ALP: Alkaline phosphatase.