Adipogenic potential of AT-MSCs is independent of donor age. Adipogenic differentiation was carried out for AT-MSCs isolated from young, adult and aged individuals. Insets show MSCs cultured in normal expansion medium. Adipogenic experiments were terminated after 21 days and oil red O was used to stain for lipid rich vacuoles, as shown (Figure 3A-C). The percentage of cells that stained positive for oil red O was determined, followed by quantification of oil red O uptake. Adipogenic differentiated MSCs isolated from young (A), adult (B) and aged (C) donors stained positive for oil red O. Differentiation levels varied between groups but non-significantly as indicated by counting oil red O positive cells (D) and colorimetrically by evaluating oil red O uptake (E). Adipogenic differentiation was further confirmed through real time RT-PCR and a non-significant difference was found when different age groups were compared (F). Results are expressed as mean ± standard deviation.