Figure 3
High-fat diet increased renal interstitial fibrosis extent and the number of vimentin positive tubules. Representative pictures (200X) of typical Sirius red staining (A) in transplanted pigs fed a normal or a high-fat diet. Histogram represents the percentage of fibrosis extent. Values significantly different from the normal diet group are represented by ***: p < 0.001. B) Representative pictures (200X) of vimentin staining revealed by diaminobenzidinine immunohistochemistry in transplanted pigs fed a normal or a high-fat diet. Arrows indicate glomerulus (Gl), tubules (Tl) and vimentin (Vim) localization. Histogram represents the quantification of the number of vimentin-positive tubules. Values significantly different from the normal diet group are represented by *: p < 0.05.