Figure 4

Responses to septic plasma measured in a PMN reporter assay for additional sets of subjects. Plasma samples from two additional sets of subjects were used in new sets of experiments: A and B corresponding to experiments II and III, respectively. PMNs used in both experiments were obtained from the same donor (donor H2) who had also participated in experiment I. Transcripts passing filter criteria of 2 fold-change and 100 difference in intensity (2FC100DIF) were used. PCA plots (left panels) were generated as described earlier. Colors and symbols indicate sample class and disease severity: red triangle, severe sepsis; green triangle, not severe sepsis; blue triangle, uninfected; and closed circle, plasma collected >48 h after admission. Numbers in parenthesis indicate percentage of variance. Box plots (right panels) show Euclidean distances calculated for each sample from the center of ellipses corresponding to responses to plasma from uninfected controls. P-values were derived from a Mann-Whitney U-test.