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Figure 2 | Journal of Translational Medicine

Figure 2

From: A transcriptomic reporter assay employing neutrophils to measure immunogenic activity of septic patients’ plasma

Figure 2

Principal component analyses of transcriptional responses to septic plasma in three different reporter cells systems. The transcriptional response to septic plasma measured by microarrays was analyzed separately for each reporter cell system. Fold-changes were calculated by normalizing the expression levels of cell cultures stimulated with plasma to their respective unstimulated cell cultures. Transcripts passing a filter criteria of 2 fold-change and 100 difference in intensity (2FC100DIF) were used for principal component analysis (PCA). Scores from principal component 1 (PC1) and principal component 2 (PC2), which explain approximately 50% of the variability, were plotted. Samples were color-coded according to study groups (blue = uninfected plasma; red = septic plasma; left panels) or cell donors (blue = donor H1; black = donor H2; right panels). Each ellipse indicates the 95% confidence interval of data from the corresponding group (indicated by colors). Numbers in parentheses indicate percentage of variance.

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