Figure 3
From: Bufalin attenuates the stage and metastatic potential of hepatocellular carcinoma in nude mice
Bufalin inhibits pulmonary metastases. (A) Detection of pulmonary metastases in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups by fluorescence microscopy and necrotic tumor tissues by H&E staining (×400). (B) Detection of intrahepatic, enteric cavity, and abdominal wall tissues by H&E staining (×200). (C) The pixel numbers of pulmonary metastatic foci in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups. (D) The cell numbers of pulmonary metastatic nodules in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups. All data represent the mean ± SD (n = 6). †P < 0.01 vs. control (NS).