Figure 2
From: Bufalin attenuates the stage and metastatic potential of hepatocellular carcinoma in nude mice
Orthotopic transplanted tumor tissues undergo necrosis and apoptosis induced by bufalin treatment. (A) Detection of transplanted tumors in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups by fluorescence microscopy and necrotic tumor tissues by H&E staining (×400). (B) Positive TUNEL staining in the nuclei of control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups (×200 and ×400). (C) TUNEL staining showed different apoptotic rates in control (NS), low-dose bufalin-treated (BF1), high-dose bufalin-treated (BF2), and LY294002-treated (LY) groups. All data represent the mean ± SD (n = 6). *P < 0.05 vs. control (NS); †P < 0.01 vs. control (NS).