Figure 5
The peripheral and local expression of sFGL2. Serum sFGL2 presented a fluctuating increase after transplantation, reached the peak at day 10 and gradually fell off eversince (A). Immunohistochemistry staining at 400× magnification showed that sFGL2 positive cells included TECs, interstitial cells and a few in the glomeruli (B). The number of sFGL2 positive cells in the kidney (C) and western blotting (D-E) revealed that there was no significant difference in the expression of sFGL2 between normal renal tissue and that of day 2 post transplantation. sFGL2 in the kidney remarkably increased from day 5, culminated at day 7 and gradually decreased at day 10 and 14. Data are expressed as mean ± SEM (n = 3). Post N: post nephrectomy. *: P < 0.05; **: P < 0.01; ***: P < 0.001.