Epigenetic inactivation of mir-34b/c in addition to mir-34a and DAPK1 in chronic lymphocytic leukemia

Table 2 Primer sequences and the reaction condition of DAPK1 and GAPDH Reverse transcription-polymerase chain reaction (RT-PCR)

Gene	Forward primer (5’ to 3’)	Reverse primer (5’ to 3’)	Tm/cycles/MgCl₂	References
DAPK1	CAGTTTGCGGTTGTGAAGAA	CCTGCAACGAGTTCCAAGAT	53°C/35×/2 mM	[31]
GAPDH	ACCACAGTCCATGCCATCACT	TCCACCACCCTGTTGCTGTA	60°C/24×/2 mM	[23]

ISSN: 1479-5876