Figure 2
Effects of oleanolic acid (OA) on nuclear/total Nrf2, Keap1, HO-1 and NQO1 expressions in chronic CsA nephropathy. (A) Representative Western blot showing the effects of OA on nuclear/total Nrf2, Keap1, HO-1 and NQO1 expression in chronic CsA nephropathy. (B) Quantitative analyses for total Nrf2/β-actin. There were no significant differences identified by quantitative analysis for immunoblotting of total Nrf2 among the experimental groups. (C) Quantitative analyses for nuclear/total Nrf2. There was increased nuclear/total Nrf2 in the CsA + OA compared with CsA. *p <0.05 vs. VH and CsA groups. (D) Quantitative analyses for Keap1/β-actin. *p <0.05 vs. other groups. (E) Quantitative analyses for HO-1/β-actin. Quantification of HO-1 immunoblotting revealed a significant increase in CsA + OA mice compared with other groups. *p <0.05 vs. other groups. (F) Quantitative analyses for NQO1/β-actin. There were no significant differences in the expression of NQO1 among the experimental groups. Nrf2 = nuclear factor erythroid-2-related factor-2; Keap1 = Kelch-like ECH-associated protein 1; HO-1 = heme oxygenase-1; NQO1 = NAD(P)H quinone oxidoreductase-1; VH = vehicle-treated group; VH + OA = vehicle plus oleanolic acid-treated group; CsA = cyclosporine-treated group; CsA + OA = cyclosporine plus oleanolic acid-treated group.